恩诺沙星检测卡
<b>产品介绍：</b><br><p><span style="font-family: 宋体; text-indent: 28px;">本试纸卡应用竞争抑制免疫层析的原理，样品中含有的恩诺沙星药物在流动过程中与胶体金标记的特异性单克隆抗体竞争性结合，从而抑制了抗体和NC膜检测线上的恩诺沙星药物-BSA偶联物的结合。</span></p><b>规格参数：</b><br><p style="margin-top:21px;text-align:center"><strong><span style="font-size:19px;font-family:黑体">恩诺沙星</span></strong><strong><span style="font-size:19px;font-family:黑体">快速检测试纸说明书</span></strong></p><p style="margin-left: 24px"><strong><span style=";font-family:宋体">1． </span></strong><strong><span style=";font-family:宋体">检测原理</span></strong></p><p style="text-indent: 28px"><span style=";font-family:宋体">本试纸卡应用竞争抑制免疫层析的原理，样品中含有的恩诺沙星药物在流动过程中与胶体金标记的特异性单克隆抗体竞争性结合，从而抑制了抗体和NC膜检测线上的恩诺沙星药物-BSA偶联物的结合。</span><span style="font-family: 宋体; text-align: center;"> </span></p><p style="margin-left: 24px"><strong><span style=";font-family:宋体">2． </span></strong><strong><span style=";font-family:宋体">检测范围</span></strong></p><p style="text-indent: 28px"><span style=";font-family:宋体">本品适用于肉类组织中的恩诺沙星药物检测</span></p><p style="margin-left: 24px"><strong><span style=";font-family:宋体">3． </span></strong><strong><span style=";font-family:宋体">检测灵敏度</span></strong></p><p><strong><span style=";font-family:宋体"> </span></strong><span style=";font-family:宋体">50ppb</span></p><p style="margin-left: 24px"><strong><span style=";font-family:宋体">4． </span></strong><strong><span style=";font-family:宋体">产品组成</span></strong></p><p><span style=";font-family:宋体"> </span><span style=";font-family:宋体">（1）1条试纸卡与1个微孔试剂放于1个铝箔袋中。</span></p><p><span style=";font-family:宋体"> </span><span style=";font-family:宋体">（2）稀释液。</span></p><p><span style=";font-family:宋体"> </span><span style=";font-family:宋体">（3）15ml离心管，1.5ml离心管。</span></p><p><span style=";font-family:宋体"> </span><span style=";font-family:宋体">（4）说明书1份。</span></p><p style="margin-left: 24px"><strong><span style=";font-family:宋体">5． </span></strong><strong><span style=";font-family:宋体">操作步骤</span></strong></p><p style="margin-left: 24px"><span style="font-family: 宋体;">（1）<span style="font-variant-numeric: normal;font-variant-east-asian: normal;font-stretch: normal;font-size: 9px;line-height: normal;font-family: 'Times New Roman'"> </span></span><span style="font-family: 宋体;">实验前请先仔细阅读操作说明书。使用前将本试纸卡和微孔试剂恢复至室温。按以下步骤</span><span style="font-family: 宋体;">操作：</span></p><p style="margin-left: 24px"><span style="font-family: 宋体;">（2）</span><span style="font-family: 宋体;">样品应当避光冷藏保存，除脂肪组织，再取切碎的组织样本，于均质机中均质</span>1min<span style="font-family: 宋体;">，称取</span>4g<span style="font-family: 宋体;">均质物置于</span>15ml<span style="font-family: 宋体;">离心管中，往离心管中加入</span>4ml<span style="font-family: 宋体;">甲醇， 充分振荡</span>5<span style="font-family: 宋体;">分钟</span>5000rpm<span style="font-family: 宋体;">离心</span>5<span style="font-family: 宋体;">分钟，取</span>3ml<span style="font-family: 宋体;">上层液体于玻璃烧杯中，电吹风吹干液体，往玻璃烧杯中加入</span>0.4ml<span style="font-family: 宋体;">稀释液，</span><span style="font-family: 宋体;">充分溶解杯底残留物，再加入</span>0.6ml<span style="font-family: 宋体;">正己烷充分混匀，取全部溶解液于</span> 1.5ml<span style="font-family: 宋体;">离心管中，</span> <span style="font-family: 宋体;">静止分层后将上层有机相去除，下层溶液即为待检液。</span></p><p style="margin-left: 24px"><span style="font-family: 宋体;">（3）从原包装中取出铝箔袋，打开后取出试纸卡与微孔试剂，并做好标记。（请在60min内尽快使用）。</span></p><p><span style=";font-family:宋体"> </span><span style=";font-family:宋体">（4）用微量移液器吸取200</span><span style="font-family:宋体">μ</span><span style=";font-family:宋体">l</span><span style=";font-family:宋体">下层溶液于微孔中，缓慢抽吸且充分与微孔中试剂混匀，</span><span style=";font-family:宋体">直至小</span><span style="font-family: 宋体;">杯底部和四壁的红色物质全部溶解，即为检测液</span><span style="font-family: 宋体;">。</span></p><p><span style="font-family: 宋体;"> （5）</span><span style="font-family: 宋体;">将标记好的试纸卡水平放置，用滴管向试卡孔缓慢逐滴加入</span>2-3<span style="font-family: 宋体;">滴不含气泡的检测液</span><span style="font-family: 宋体;">。</span></p><p><span style="font-family: 宋体;"> （6）再次室温（20-25℃）孵育5min，取出试纸条并根据示意图判定结果，其他时间判定无效。</span></p><p style="margin-left: 24px"><strong><span style=";font-family:宋体">6． </span></strong><strong><span style=";font-family:宋体">结果判定</span></strong></p><p><img src="/upload/u/6/202103/16152518645199.jpg" alt="瘦肉精检测卡|瘦肉精三联检测卡|克伦检测|莱克检测|沙丁检测|宠物试纸|犬瘟试纸|细小试纸|宠物试纸代加工|宠物试纸批发|动物扑杀器|消毒液机|一次性防护服|动物解剖台|采样箱|检疫箱|检疫设备"/></p><p style="margin-left:42px"><span style=";font-family:宋体">阴性：</span>C<span style=";font-family:宋体">线显色，</span>T<span style=";font-family:宋体">线肉眼可见，无论颜色深浅均判为阴性。</span></p><p style="margin-left:42px"><span style=";font-family:宋体">阳性：</span>C<span style=";font-family:宋体">线显色，</span>T<span style=";font-family:宋体">线不显色，或</span>T<span style=";font-family:宋体">线隐隐约约，判为阳性。</span></p><p style="margin-left:42px"><span style=";font-family:宋体">无效：</span>C<span style=";font-family:宋体">线不显色，无论</span>T<span style=";font-family:宋体">线是否显色，该试纸均判为无效。</span></p><p style="margin-left: 24px"><strong><span style=";font-family:宋体">7． </span></strong><strong><span style=";font-family:宋体">特异性</span></strong></p><p style="text-indent: 28px"><span style=";font-family: 宋体">检测500</span><span style="font-family:宋体">μg/L的磺胺类、氯毒素类、氨基糖苷类、β-内酰胺类和四环素类等药物，结果均呈阴性。</span></p><p style="margin-left: 24px"><strong><span style=";font-family:宋体">8． </span></strong><strong><span style=";font-family:宋体">储存及有效期</span></strong></p><p style="text-indent: 28px"><span style=";font-family:宋体">存放于2-30℃阴凉避光干燥处，切勿冷冻，有效期为18个月，批号和有效期见包装盒。</span></p><p style="margin-left: 24px"><strong><span style=";font-family:宋体">9． </span></strong><strong><span style=";font-family:宋体">注意事项</span></strong></p><p><span style=";font-family:宋体"> (1)</span><span style=";font-family:宋体">请按照操作步骤进行测试，操作时请勿触摸试纸条显色区。</span></p><p><span style=";font-family:宋体"> (2)</span><span style=";font-family:宋体">不要混用不同批号的试纸条和微孔试剂。</span></p><p><span style=";font-family:宋体"> (3)</span><span style=";font-family:宋体">本试纸条为一次性产品，请勿重复使用。</span></p><p style="text-indent: 28px"><span style=";font-family:宋体">(4)</span><span style=";font-family:宋体">本产品检测结果仅供参考，如需确证，请参照国家相关标准方法。</span></p><p style="text-indent: 28px"><span style=";font-family:宋体"> </span></p><p><strong><span style=";font-family:宋体"> </span></strong></p><p><strong><span style=";font-family:宋体"> </span></strong></p><p><strong><span style=";font-family:宋体"> </span></strong></p><p><strong><span style=";font-family:宋体"> </span></strong></p><p><strong><span style=";font-family:宋体"> </span></strong></p><p> </p><p><br/></p><b>包装：</b><br><p>25条/盒 50盒/箱</p>
