孔雀石绿检测卡
<p><strong>产品介绍：</strong><br/></p><p><span style="font-size: 12px; font-family: 宋体;">孔雀石绿快速检测试纸基于胶体金免疫层析技术。检测时，样品中的孔雀石绿在流动过程中与胶体金标记的特异性抗体结合，抑制了抗体与固相载体膜上的孔雀石绿</span><span style="font-size: 12px;">-BSA</span><span style="font-size: 12px; font-family: 宋体;">偶联物的结合，如果样品中的孔雀石绿含量大于灵敏度，检测线在规定时间内不显色或非常模糊时，结果为阳性，反之，检测线接近</span><span style="font-size: 12px;">C</span><span style="font-size: 12px; font-family: 宋体;">线或者深于</span><span style="font-size: 12px;">C</span><span style="font-size: 12px; font-family: 宋体;">线时，结果为阴性。无论检测液中孔雀石绿浓度高低，</span><span style="font-size: 12px;">C</span><span style="font-size: 12px; font-family: 宋体;">线均显红色色带。</span></p><p><strong>规格参数：</strong><br/></p><p style="margin-left:28px"><span style=";font-family:Wingdings">l<span style="font-variant-numeric: normal;font-variant-east-asian: normal;font-stretch: normal;font-size: 9px;line-height: normal;font-family: 'Times New Roman'"> </span></span><strong><span style=";font-family:黑体">原理</span></strong></p><p><span style="font-size:12px;font-family:宋体">孔雀石绿快速检测试纸基于胶体金免疫层析技术。检测时，样品中的孔雀石绿在流动过程中与胶体金标记的特异性抗体结合，抑制了抗体与固相载体膜上的孔雀石绿</span><span style="font-size:12px">-BSA</span><span style="font-size:12px;font-family:宋体">偶联物的结合，如果样品中的孔雀石绿含量大于灵敏度，检测线在规定时间内不显色或非常模糊时，结果为阳性，反之，检测线接近</span><span style="font-size:12px">C</span><span style="font-size:12px;font-family:宋体">线或者深于</span><span style="font-size:12px">C</span><span style="font-size:12px;font-family:宋体">线时，结果为阴性。无论检测液中孔雀石绿浓度高低，</span><span style="font-size:12px">C</span><span style="font-size:12px;font-family:宋体">线均显红色色带。</span></p><p style="margin-left:28px"><span style=";font-family:Wingdings">l<span style="font-variant-numeric: normal;font-variant-east-asian: normal;font-stretch: normal;font-size: 9px;line-height: normal;font-family: 'Times New Roman'"> </span></span><strong><span style=";font-family:黑体">使用范围</span></strong></p><p><span style="font-size:12px;font-family:宋体">用于快速筛查水产品组织样本中的孔雀石绿。</span></p><p style="margin-left:28px"><span style=";font-family:Wingdings">l<span style="font-variant-numeric: normal;font-variant-east-asian: normal;font-stretch: normal;font-size: 9px;line-height: normal;font-family: 'Times New Roman'"> </span></span><strong><span style=";font-family:黑体">灵敏度</span></strong></p><table cellspacing="0" cellpadding="0"><tbody><tr style=";height:18px" class="firstRow"><td width="170" valign="top" style="border-width: 1px; border-color: black; padding: 0px;" height="18"><p style="margin-left: 7px;line-height: 15px"><span style="font-size: 12px;font-family:宋体;position:relative;top:1px">名称</span></p></td><td width="170" valign="top" style="border-top-width: 1px; border-right-width: 1px; border-bottom-width: 1px; border-top-color: black; border-right-color: black; border-bottom-color: black; border-left: none; padding: 0px;" height="18"><p style="margin-left: 7px;line-height: 16px"><span style="font-size: 12px;font-family:宋体;position:relative;top:1px">灵敏度<span style="letter-spacing:-0">（</span></span><span style="font-size:12px;position:relative;top:1px;letter-spacing:-0">p</span><span style="font-size:12px;position:relative;top:1px;letter-spacing:0">p</span><span style="font-size:12px;position:relative;top:1px;letter-spacing:-0">b</span><span style="font-size:12px;font-family:宋体;position:relative;top:1px">）</span></p></td></tr><tr style=";height:18px"><td width="170" valign="top" style="border-right-width: 1px; border-bottom-width: 1px; border-left-width: 1px; border-right-color: black; border-bottom-color: black; border-left-color: black; border-top: none; padding: 0px;" height="18"><p style="margin-left: 7px;line-height: 15px"><span style="font-size: 12px;font-family:宋体;position:relative;top:1px">孔雀石<span style="letter-spacing:-0">绿</span>（隐性）</span></p></td><td width="170" valign="top" style="border-top: none; border-left: none; border-bottom-width: 1px; border-bottom-color: black; border-right-width: 1px; border-right-color: black; padding: 0px; word-break: break-all;" height="18"><p style="margin-left: 7px;line-height: 15px"><span style="font-size:12px">1</span></p></td></tr></tbody></table><p style="margin-left:28px"><span style=";font-family:Wingdings"><br/>l<span style="font-variant-numeric: normal;font-variant-east-asian: normal;font-stretch: normal;font-size: 9px;line-height: normal;font-family: 'Times New Roman'"> </span></span><strong><span style=";font-family:黑体">保存和稳定性</span></strong></p><p><span style="font-size:12px;font-family:宋体">试纸卡于室温</span><span style="font-size:12px">(<30</span><span style="font-size:12px;font-family:宋体">℃</span><span style="font-size:12px">)</span><span style="font-size:12px;font-family:宋体">阴凉干燥处保存，<span style="color:red">不可冷冻</span>，避免阳光直晒，自生产日期起</span><span style="font-size:12px">12</span><span style="font-size:12px;font-family:宋体">个月内有效。</span></p><p style="margin-left:28px"><span style=";font-family:Wingdings">l<span style="font-variant-numeric: normal;font-variant-east-asian: normal;font-stretch: normal;font-size: 9px;line-height: normal;font-family: 'Times New Roman'"> </span></span><strong><span style=";font-family:黑体">测试步骤 </span></strong></p><p style="margin-left: 18px"><span style="font-size:12px;font-family:宋体">【样品处理】组织样品（虾要去掉头和壳后彻底清洗干净，鱼要去鳞后洗干净）应当避光冷藏保存。</span></p><p style="margin-left: 18px"><span style="font-size:12px;font-family:宋体">样品的处理方法如下：</span></p><p style="margin-left: 18px"><span style="font-size:12px">1. </span><span style="font-size:12px;font-family:宋体">取切碎的一定量的去脂肪组织样本，用均质机均质；</span></p><p style="margin-left: 18px"><span style="font-size:12px">2. </span><span style="font-size:12px;font-family:宋体">称取</span><span style="font-size:12px">5g</span><span style="font-size:12px;font-family:宋体">均质物于</span><span style="font-size:12px">50ml</span><span style="font-size:12px;font-family:宋体">离心管中；</span></p><p style="margin-left: 18px"><span style="font-size:12px">3. </span><span style="font-size:12px;font-family:宋体">向上述</span><span style="font-size:12px">50ml</span><span style="font-size:12px;font-family:宋体">管中加入提取剂</span><span style="font-size:12px">1</span><span style="font-size:12px;font-family:宋体">溶液</span><span style="font-size:12px">3ml</span><span style="font-size:12px;font-family:宋体">，然后加入</span><span style="font-size:12px">8ml</span><span style="font-size:12px;font-family:宋体">乙腈，再加入一瓶提取剂</span><span style="font-size:12px">2</span><span style="font-size:12px;font-family:宋体">，盖上盖子剧烈振荡</span><span style="font-size:12px">2min</span><span style="font-size:12px;font-family:宋体">后，室温下</span><span style="font-size:12px">4000rpm</span><span style="font-size:12px;font-family:宋体">离心</span><span style="font-size:12px">5min</span><span style="font-size:12px;font-family:宋体">；</span></p><p style="margin-left: 18px"><span style="font-size:12px">4. </span><span style="font-size:12px;font-family:宋体">移取</span><span style="font-size:12px">4ml</span><span style="font-size:12px;font-family:宋体">上清液于</span><span style="font-size:12px">5ml</span><span style="font-size:12px;font-family:宋体">离心管中，加入</span><span style="font-size:12px">100ul</span><span style="font-size:12px;font-family:宋体">氧化剂，盖上盖子颠倒混匀</span><span style="font-size:12px">10</span><span style="font-size:12px;font-family:宋体">秒钟，于</span><span style="font-size:12px">60</span><span style="font-size:12px;font-family:宋体">℃环境下，利用氮气或空气吹干。</span></p><p style="margin-left: 18px"><span style="font-size:12px">5. </span><span style="font-size:12px;font-family:宋体">向吹干的离心管中加入</span><span style="font-size:12px">0.3mlMG</span><span style="font-size:12px;font-family:宋体">复溶液，和正己烷</span><span style="font-size:12px">0.5ml,</span><span style="font-size:12px;font-family:宋体">于室温下</span><span style="font-size:12px">4000rpm</span><span style="font-size:12px;font-family:宋体">离心</span><span style="font-size:12px">1min</span><span style="font-size:12px;font-family:宋体">，用移液器移取下层</span><span style="font-size:12px">150</span><span style="font-size:12px;font-family:宋体">μ</span><span style="font-size:12px">l</span><span style="font-size:12px;font-family:宋体">溶液，待检。</span></p><p style="margin-left:18px"><span style="font-size:12px;font-family:宋体">【使用步骤】测试前先完整阅读使用说明书，使用前将金标条和待检样本溶液恢复至室温（</span><span style="font-size:12px">20</span><span style="font-size:12px;font-family:宋体">℃</span><span style="font-size:12px">~30</span><span style="font-size:12px;font-family:宋体">℃）。</span></p><p style="margin-left:18px"><span style="font-size:12px">1. </span><span style="font-size:12px;font-family:宋体">从原包装袋中取出金标条，水平放置于观察者正面；</span></p><p style="margin-left:18px"><span style="font-size:12px">2. </span><span style="font-size:12px;font-family:宋体">吸取待检样品</span><span style="font-size:12px">150</span><span style="font-size:12px;font-family:宋体">微升加入到金标微孔，吹打完全溶解孔内红色物质，反应</span><span style="font-size:12px">5</span><span style="font-size:12px;font-family:宋体">分钟；</span></p><p style="margin-left:18px"><span style="font-size:12px">3. </span><span style="font-size:12px;font-family:宋体">吸取孔内所有溶液滴加到加样孔中，加样后开始计时；</span></p><p style="margin-left:18px"><span style="font-size:12px">4. </span><span style="font-size:12px;font-family:宋体">结果应在</span><span style="font-size:12px">5</span><span style="font-size:12px;font-family:宋体">～</span><span style="font-size:12px">8</span><span style="font-size:12px;font-family:宋体">分钟读取，其他时间判读无效。</span></p><p style="margin-left:18px"><span style="font-size:12px">5. T</span><span style="font-size:12px;font-family:宋体">线明显显为绿色的，可判为阳性结果</span></p><p style="margin-left:18px"><span style="font-size:12px"> </span></p><p style="margin-left:28px"><span style=";font-family:Wingdings">l<span style="font-variant-numeric: normal;font-variant-east-asian: normal;font-stretch: normal;font-size: 9px;line-height: normal;font-family: 'Times New Roman'"> </span></span><strong><span style=";font-family:黑体">结果判断</span></strong></p><p><img src="/upload/u/6/202103/16152518637023.jpg" alt="瘦肉精检测卡|瘦肉精三联检测卡|克伦检测|莱克检测|沙丁检测|宠物试纸|犬瘟试纸|细小试纸|宠物试纸代加工|宠物试纸批发|动物扑杀器|消毒液机|一次性防护服|动物解剖台|采样箱|检疫箱|检疫设备"/></p><p style="margin-left:24px"><span style="font-size:12px;font-family:宋体">阴性：</span><span style="font-size:12px">T</span><span style="font-size:12px;font-family:宋体">线显色（测试线，靠近加样孔一端）比</span><span style="font-size:12px">C</span><span style="font-size:12px;font-family:宋体">线（对照线）</span></p><p style="margin-left:24px"><span style="font-size:12px"> </span><span style="font-size:12px;font-family:宋体">深或一样深，表示样品中待检药物浓度低于</span><span style="font-size:12px">1ppb</span><span style="font-size:12px;font-family:宋体">或不</span></p><p style="margin-left:24px"><span style="font-size:12px"> </span><span style="font-size:12px;font-family:宋体">含待检药物。</span></p><p style="margin-left:24px"><span style="font-size:12px;font-family:宋体">阳性：</span><span style="font-size:12px">T</span><span style="font-size:12px;font-family:宋体">线显色比</span><span style="font-size:12px">C</span><span style="font-size:12px;font-family:宋体">线浅，或</span><span style="font-size:12px">T</span><span style="font-size:12px;font-family:宋体">线无显色，表示样品中待检药</span><span style="font-size:12px"> </span></p><p style="margin-left:24px"><span style="font-size:12px"> </span><span style="font-size:12px;font-family:宋体">物浓度高于</span><span style="font-size:12px">1ppb</span><span style="font-size:12px;font-family:宋体">，</span><span style="font-size:12px">T</span><span style="font-size:12px;font-family:宋体">线相比</span><span style="font-size:12px">C</span><span style="font-size:12px;font-family:宋体">线越浅，表示样品中待检</span></p><p style="margin-left:24px"><span style="font-size:12px"> </span><span style="font-size:12px;font-family:宋体">药物浓度越高。</span></p><p style="margin-left:24px"><span style="font-size:12px;font-family:宋体">无效：未出现</span><span style="font-size:12px">C</span><span style="font-size:12px;font-family:宋体">线，表明不正确的操作过程或试剂板已失效。</span></p><p style="margin-left:24px"><span style="font-size:12px"> </span><span style="font-size:12px;font-family:宋体">应再次仔细阅读说明书，并用新的试剂板重新测试。</span></p><p style="margin-left:28px"><span style=";font-family:Wingdings">l<span style="font-variant-numeric: normal;font-variant-east-asian: normal;font-stretch: normal;font-size: 9px;line-height: normal;font-family: 'Times New Roman'"> </span></span><strong><span style=";font-family:黑体">注意事项</span></strong></p><p style="margin-left:28px"><span style="font-size: 12px;">1. </span><span style="font-size: 12px; font-family: 宋体;">尽量不要触摸金标条中央的白色膜面；</span></p><p><span style="font-size:12px"> 2. </span><span style="font-size:12px;font-family:宋体">请勿使用过期的金标条；</span></p><p><span style="font-size:12px"> 3. </span><span style="font-size:12px;font-family:宋体">若需直接检测标准品，请用我方提供的</span><span style="font-size:12px">PBST</span><span style="font-size:12px;font-family:宋体">缓冲液进行配制。切勿重复使用配备的滴管，以免交叉污染；</span></p><p><span style="font-size:12px"> 4. </span><span style="font-size:12px;font-family:宋体">切勿食用配备的试剂；</span></p><p><span style="font-size:12px"> 5. </span><span style="font-size:12px;font-family:宋体">试验中务必戴上配备一次性手套；</span></p><p style="margin-left: 18px"><span style="font-size:12px"> 6. </span><span style="font-size:12px;font-family:宋体">试验场地要求常温</span><span style="font-size:12px">20</span><span style="font-size:12px;font-family:宋体">℃</span><span style="font-size:12px">-30</span><span style="font-size:12px;font-family:宋体">℃的通风条件</span></p><p> </p><p style="margin-left:28px"><span style=";font-family:Wingdings">l<span style="font-variant-numeric: normal;font-variant-east-asian: normal;font-stretch: normal;font-size: 9px;line-height: normal;font-family: 'Times New Roman'"> </span></span><strong><span style=";font-family:黑体">特异性</span></strong></p><p><span style="font-size: 12px;font-family:宋体">本产品与其他种类药物无交叉反应。</span></p><p style="margin-left:28px"><span style=";font-family:Wingdings">l<span style="font-variant-numeric: normal;font-variant-east-asian: normal;font-stretch: normal;font-size: 9px;line-height: normal;font-family: 'Times New Roman'"> </span></span><strong><span style=";font-family:黑体">精密度</span></strong></p><p style="margin: 0 5px 0 27px;line-height: 17px"><span style="font-size:12px;font-family:宋体">同时使用本产品和孔雀石绿液相质谱串联质谱法对</span></p><p style="line-height: 17px"><span style="font-size:12px;font-family:宋体">对</span><span style="font-size:12px">150</span><span style="font-size:12px;font-family:宋体">份样品包括</span><span style="font-size:12px">94</span><span style="font-size:12px;font-family:宋体">份阴性样本和</span><span style="font-size:12px">56</span><span style="font-size:12px;font-family:宋体">份阳性样本进行检测，检测结果表明本产品与质谱法的结果符合率为</span><span style="font-size:12px">98.0</span><span style="font-size:12px;font-family:宋体">％。</span></p><p style="line-height: 17px"><span style="font-size:12px"> </span></p><p style="margin-left:28px"><br/></p><p> </p><p><span style="font-size:12px;font-family:宋体"> </span></p><p><br/></p><p><strong>包装：</strong><br/></p><p>25条/盒 </p><p>50盒/箱</p>
