呋喃唑酮代谢物 (AOZ)检测卡

<b>产品介绍：</b><br><p><span style="font-family: 宋体; font-size: 12px;">呋喃唑酮代谢物 (AOZ)快速检测试纸基于胶体金免疫层析技术。检测时，样品中的AOZ在流动过程中与胶体金标记的特异性抗体结合，抑制了抗体与固相载体膜上的AOZ-OVA偶联物的结合，如果样品中的AOZ含量大于检测灵敏度，T线在规定时间内不显颜色，结果为阳性，反之，T线显红色色带，结果为阴性。无论检测液中AOZ浓度高低，C线均显红色色带。</span></p><b>规格参数：</b><br><p style="line-height:21px;text-autospace:none;vertical-align:bottom"><strong><span style="font-size:19px;font-family:&#39;Arial&#39;,sans-serif">&nbsp;</span></strong></p><p style="text-align:center;line-height:16px"><span style="font-size:16px;font-family:黑体">呋喃唑酮代谢物 (AOZ)</span><span style="font-size:16px;font-family:黑体">快速检测试纸卡使用说明书</span></p><p><strong>&nbsp;</strong></p><p style="margin-left:28px"><span style=";font-family:Wingdings">l<span style="font-variant-numeric: normal;font-variant-east-asian: normal;font-stretch: normal;font-size: 9px;line-height: normal;font-family: &#39;Times New Roman&#39;">&nbsp; </span></span><strong><span style=";font-family:黑体">原理</span></strong></p><p style="line-height: 21px;vertical-align: bottom"><span style="font-size:12px;font-family:宋体">呋喃唑酮代谢物 (AOZ)快速检测试纸基于胶体金免疫层析技术。检测时，样品中的AOZ在流动过程中与胶体金标记的特异性抗体结合，抑制了抗体与固相载体膜上的AOZ-OVA偶联物的结合，如果样品中的AOZ含量大于检测灵敏度，T线在规定时间内不显颜色，结果为阳性，反之，T线显红色色带，结果为阴性。无论检测液中AOZ浓度高低，C线均显红色色带。</span></p><p style="margin-left:28px"><span style=";font-family:Wingdings">l<span style="font-variant-numeric: normal;font-variant-east-asian: normal;font-stretch: normal;font-size: 9px;line-height: normal;font-family: &#39;Times New Roman&#39;">&nbsp; </span></span><strong><span style=";font-family:黑体">使用范围</span></strong></p><p><span style="font-size:12px;font-family:宋体">用于快速筛查鱼虾中的呋喃唑酮（AOZ）。</span></p><p style="margin-left:28px"><span style=";font-family:Wingdings">l<span style="font-variant-numeric: normal;font-variant-east-asian: normal;font-stretch: normal;font-size: 9px;line-height: normal;font-family: &#39;Times New Roman&#39;">&nbsp; </span></span><strong><span style=";font-family:黑体">灵敏度</span></strong></p><p><span style="font-size:12px;font-family:宋体">鱼虾：1ppb</span></p><p style="margin-left:28px"><span style=";font-family:Wingdings">l<span style="font-variant-numeric: normal;font-variant-east-asian: normal;font-stretch: normal;font-size: 9px;line-height: normal;font-family: &#39;Times New Roman&#39;">&nbsp; </span></span><strong><span style=";font-family:黑体">保存和稳定性</span></strong></p><p><span style="font-size:12px;font-family:宋体">试纸卡于室温(&lt;30℃)阴凉干燥处保存，不可冷冻，避免阳光直晒，生产日期起18个月内有效。</span></p><p style="margin-left:28px"><span style=";font-family:Wingdings">l<span style="font-variant-numeric: normal;font-variant-east-asian: normal;font-stretch: normal;font-size: 9px;line-height: normal;font-family: &#39;Times New Roman&#39;">&nbsp; </span></span><strong><span style=";font-family:黑体">测试步骤</span></strong></p><p style="line-height:21px;text-autospace:none;vertical-align: bottom"><span style="font-size:12px;font-family:宋体">衍生反应 </span></p><p style=";line-height: 17px"><span style="font-size:12px;font-family:宋体">1. a.</span> <span style="font-size: 13px;font-family:宋体">称</span><span style="font-size:12px;font-family:宋体">取2 g已破碎搅匀的待测样品放入50 mL离心管中，加入4 mL H2O，0.5 mL 1N HCl，200μL 衍生试剂。</span></p><p style=";line-height: 17px"><span style="font-size: 12px; font-family: 宋体;">2. </span><span style="font-size: 12px; font-family: 宋体;">振荡混合约3分钟，60℃水浴条件下孵育 1h。</span></p><p style="line-height:21px;text-autospace:none;vertical-align: bottom"><span style="font-size:12px;font-family:宋体">萃取流程</span></p><p style="line-height:21px;text-autospace:none;vertical-align: bottom"><span style="font-size: 12px; font-family: 宋体;">1. </span><span style="font-size: 12px; font-family: 宋体;">加入5 mL 0.1M 磷酸氢二钾溶液，0.4 mL 1N NaOH，6 mL乙酸乙酯 （Ethyl acetate）。</span></p><p style="line-height:21px;text-autospace:none;vertical-align: bottom"><span style="text-indent: 18px; font-size: 12px; font-family: 宋体;">2. </span><span style="text-indent: 18px; font-size: 12px; font-family: 宋体;">振荡混合约3分钟，离心机离心5分钟（4000 rpm）。</span></p><p style="line-height:21px;text-autospace:none;vertical-align: bottom"><span style="text-indent: 18px; font-size: 12px; font-family: 宋体;">3. </span><span style="text-indent: 18px; font-size: 12px; font-family: 宋体;">取 3 mL上层液 （乙酸乙酯层） 至5 ml离心管。</span></p><p style="line-height:21px;text-autospace:none;vertical-align: bottom"><span style="font-size: 12px; font-family: 宋体;">4. </span><span style="font-size: 12px; font-family: 宋体;">利用氮吹仪（在60℃下）或电吹风（不要用强热风）将乙酸乙酯吹干。</span></p><p style="line-height:21px;text-autospace:none;vertical-align: bottom"><span style="font-size: 12px; font-family: 宋体;">5. </span><span style="font-size: 12px; font-family: 宋体;">然后加入 0.5 mL正己烷（n-Hexane）将残余物完全溶解，再加入 0.5 mL 1X sample buffer。</span></p><p style="line-height:21px;text-autospace:none;vertical-align: bottom"><span style="font-size: 12px; font-family: 宋体;">6. </span><span style="font-size: 12px; font-family: 宋体;">振荡混合约1分钟，离心机离心1分钟（4000 rpm） (若不分层则振荡混合后于沸水加热3分钟再离心一次) 。</span></p><p style="line-height:21px;text-autospace:none;vertical-align: bottom"><span style="font-size: 12px; font-family: 宋体;">7. </span><span style="font-size: 12px; font-family: 宋体;">取出下层液（水层）滴两滴在恢复室温的测试卡加样</span><span style="font-family: 宋体; font-size: 12px;">孔，5分钟判断结果。</span><span style="font-family: 宋体; font-size: 12px;">&nbsp;</span></p><p style="margin-left:28px"><span style=";font-family:Wingdings">l<span style="font-variant-numeric: normal;font-variant-east-asian: normal;font-stretch: normal;font-size: 9px;line-height: normal;font-family: &#39;Times New Roman&#39;">&nbsp; </span></span><strong><span style=";font-family:黑体">结果判断</span></strong></p><p style="margin-left:36px"><span style="font-size:12px;font-family:宋体">&nbsp;&nbsp;&nbsp;</span><span style="font-size: 12px;font-family:宋体">阴性：C线显色，T线肉眼可见，无论颜色深浅均判为阴性。</span></p><p style="margin-left:36px"><span style="font-size:12px;font-family:宋体">&nbsp;&nbsp; </span><span style="font-size: 12px;font-family:宋体">阳性：C线显色，T线不显色，或者T线隐隐约约，判为阳性。</span></p><p style="margin-left:36px"><span style="font-size:12px;font-family:宋体">&nbsp;&nbsp; </span><span style="font-size: 12px;font-family:宋体">无效：C线不显色，无论T线是否显色，该试纸均判为无效。</span></p><p style="margin-left:36px"><img src="/upload/u/6/202103/16152518633246.png" alt="瘦肉精检测卡|瘦肉精三联检测卡|克伦检测|莱克检测|沙丁检测|宠物试纸|犬瘟试纸|细小试纸|宠物试纸代加工|宠物试纸批发|动物扑杀器|消毒液机|一次性防护服|动物解剖台|采样箱|检疫箱|检疫设备|宠物用品|宠物食品|狗粮|猫粮"/></p><p style="margin-left:28px"><span style=";font-family:Wingdings">l<span style="font-variant-numeric: normal;font-variant-east-asian: normal;font-stretch: normal;font-size: 9px;line-height: normal;font-family: &#39;Times New Roman&#39;">&nbsp; </span></span><strong><span style=";font-family:黑体">注意事项</span></strong></p><p style="margin-left:18px"><span style="font-size:12px;font-family:宋体">1</span><span style="font-size:12px;font-family:宋体">．如果购买时发现过期、破损、污染的产品，请在购买处进行更换。</span></p><p style="margin-left:18px"><span style="font-size:12px;font-family:宋体">2</span><span style="font-size:12px;font-family:宋体">．所有试纸启封后1小时内使用。</span></p><p style="margin-left:18px"><span style="font-size:12px;font-family:宋体">3</span><span style="font-size:12px;font-family:宋体">．本品为一次性产品，请勿重复使用。</span></p><p style="margin-left:18px"><span style="font-size:12px;font-family:宋体">4</span><span style="font-size:12px;font-family:宋体">．本品不需要放入冰箱冷藏，室温保存即可。</span></p><p style="margin-left:18px"><span style="font-size:12px;font-family:宋体">5</span><span style="font-size:12px;font-family:宋体">．请勿使用自来水、纯化水及蒸馏水作为阴性对照。</span></p><p style="margin-left:18px"><span style="font-size:12px;font-family:宋体">6</span><span style="font-size:12px;font-family:宋体">．如滴加检测液后30秒内，在测试窗口无液体移行，则再补加1滴检测液。</span></p><p style="margin-left:18px"><span style="font-size:12px;font-family:宋体">7</span><span style="font-size:12px;font-family:宋体">．本品为筛选试剂，任何可疑及阳性结果请用其它方法作进一步确认。</span></p><p><span style="font-family:黑体"><span style="font-size: 19px;"><strong>&nbsp; &nbsp;</strong><span style="font-family: 宋体, SimSun; font-size: 12px;">包装：25条/盒</span></span></span></p><p><span style="font-size:12px;font-family:宋体">&nbsp;</span></p><p><br/></p><b>包装：</b><br><p>25条/盒&nbsp;&nbsp;</p><p>50盒/箱</p>